psin4 pamcherry plasmid (Addgene inc)
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Addgene inc
psin4 pamcherry plasmid
Psin4 Pamcherry Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/psin4+pamcherry+plasmid/pSin-EF2-LIN28-Pur+(Plasmid+%2316580)/pmc06984454-410-28-21
Average 93 stars, based on 38 article reviews
Psin4 Pamcherry Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/psin4+pamcherry+plasmid/pSin-EF2-LIN28-Pur+(Plasmid+%2316580)/pmc06984454-410-28-21
Average 93 stars, based on 38 article reviews
psin4 pamcherry plasmid - by Bioz Stars,
2026-10
93/100 stars
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Plasmid Preparation:Article Title: Age-dependent accumulation of oligomeric SNCA/α-synuclein from impaired degradation in mutant LRRK2 knockin mouse model of Parkinson disease: role for therapeutic activation of chaperone-mediated autophagy (CMA) Article Snippet: To develop WT and LRRK2 R1441G KI mutant MEFs to stably express photoactivatable PAmCherry-SNCA-NE or PAmCherry-KFERQ-NE using lentivirus, PAmCherry1 gene was amplified by PCR from pPAmCherry1-N1 plasmid, a gift from Vladislav Verkhusha (Addgene™ plasmid, 31928) [ ], as template to generate an insert including EcoRI at the 5ʹ end and NheI-PmeI at the 3ʹ-end. .. This cDNA insert of PAmCherry1 was then sub-cloned into a lentiviral backbone plasmid pSIN4-EF2-IRES-Pur derived from a gift from James Thomson (Addgene™ plasmid, Article Title: Age-dependent accumulation of oligomeric SNCA/α-synuclein from impaired degradation in mutant LRRK2 knockin mouse model of Parkinson disease: role for therapeutic activation of chaperone-mediated autophagy (CMA). Article Snippet: Cloning of lentiviral expression constructs To develop WT and LRRK2R1441G KI mutant MEFs to stably express photoactivatable PAmCherry-SNCA-NE or PAmCherry-KFERQ-NE using lentivirus, PAmCherry1 gene was amplified by PCR from pPAmCherry1-N1 plasmid, a gift from Vladislav Verkhusha (Addgene™ plasmid, 31928) [70], as template to generate an insert including EcoRI at the 5ʹ end and NheI-PmeI at the 3ʹ-end. .. This cDNA insert of PAmCherry1 was then sub-cloned into a lentiviral backbone plasmid pSIN4-EF2IRES-Pur derived from a gift from James Thomson (Addgene™ plasmid, Derivative Assay:Article Title: Age-dependent accumulation of oligomeric SNCA/α-synuclein from impaired degradation in mutant LRRK2 knockin mouse model of Parkinson disease: role for therapeutic activation of chaperone-mediated autophagy (CMA) Article Snippet: To develop WT and LRRK2 R1441G KI mutant MEFs to stably express photoactivatable PAmCherry-SNCA-NE or PAmCherry-KFERQ-NE using lentivirus, PAmCherry1 gene was amplified by PCR from pPAmCherry1-N1 plasmid, a gift from Vladislav Verkhusha (Addgene™ plasmid, 31928) [ ], as template to generate an insert including EcoRI at the 5ʹ end and NheI-PmeI at the 3ʹ-end. .. This cDNA insert of PAmCherry1 was then sub-cloned into a lentiviral backbone plasmid pSIN4-EF2-IRES-Pur derived from a gift from James Thomson (Addgene™ plasmid, Article Title: Age-dependent accumulation of oligomeric SNCA/α-synuclein from impaired degradation in mutant LRRK2 knockin mouse model of Parkinson disease: role for therapeutic activation of chaperone-mediated autophagy (CMA). Article Snippet: Cloning of lentiviral expression constructs To develop WT and LRRK2R1441G KI mutant MEFs to stably express photoactivatable PAmCherry-SNCA-NE or PAmCherry-KFERQ-NE using lentivirus, PAmCherry1 gene was amplified by PCR from pPAmCherry1-N1 plasmid, a gift from Vladislav Verkhusha (Addgene™ plasmid, 31928) [70], as template to generate an insert including EcoRI at the 5ʹ end and NheI-PmeI at the 3ʹ-end. .. This cDNA insert of PAmCherry1 was then sub-cloned into a lentiviral backbone plasmid pSIN4-EF2IRES-Pur derived from a gift from James Thomson (Addgene™ plasmid, Construct:Article Title: Age-dependent accumulation of oligomeric SNCA/α-synuclein from impaired degradation in mutant LRRK2 knockin mouse model of Parkinson disease: role for therapeutic activation of chaperone-mediated autophagy (CMA) Article Snippet: To develop WT and LRRK2 R1441G KI mutant MEFs to stably express photoactivatable PAmCherry-SNCA-NE or PAmCherry-KFERQ-NE using lentivirus, PAmCherry1 gene was amplified by PCR from pPAmCherry1-N1 plasmid, a gift from Vladislav Verkhusha (Addgene™ plasmid, 31928) [ ], as template to generate an insert including EcoRI at the 5ʹ end and NheI-PmeI at the 3ʹ-end. .. This cDNA insert of PAmCherry1 was then sub-cloned into a lentiviral backbone plasmid pSIN4-EF2-IRES-Pur derived from a gift from James Thomson (Addgene™ plasmid, Article Title: Age-dependent accumulation of oligomeric SNCA/α-synuclein from impaired degradation in mutant LRRK2 knockin mouse model of Parkinson disease: role for therapeutic activation of chaperone-mediated autophagy (CMA). Article Snippet: Cloning of lentiviral expression constructs To develop WT and LRRK2R1441G KI mutant MEFs to stably express photoactivatable PAmCherry-SNCA-NE or PAmCherry-KFERQ-NE using lentivirus, PAmCherry1 gene was amplified by PCR from pPAmCherry1-N1 plasmid, a gift from Vladislav Verkhusha (Addgene™ plasmid, 31928) [70], as template to generate an insert including EcoRI at the 5ʹ end and NheI-PmeI at the 3ʹ-end. .. This cDNA insert of PAmCherry1 was then sub-cloned into a lentiviral backbone plasmid pSIN4-EF2IRES-Pur derived from a gift from James Thomson (Addgene™ plasmid, |